The current publication of the results of the main author Josef Lazar, Ph.D., is the result of a ten-year effort begun at Columbia University in the USA and pursued his team at the Institute of Structural Biology and nanobiology CVGZ Academy of Sciences, the University of South Bohemia – Faculty and Institute of Physical Biology JU in Hrady.
University – Faculty of Science and Institute of Physical Biology JU in Hrady. The new microscope will help to discover new drugs and to understand the process of functioning of cells and whole organisms. As a particularly interesting area of application of the new microscope appears to be able to see the electrical signals traveling in individual neurons in the brain. Co-author publications Alexey Bondar is a student of South Bohemian University, Department of Physical Biology.
** The newly developed microscope allows the monitoring of molecular processes in cells **
Josef Lazar, Alexey Bondar, Stephen Timrå, Stuart Firestein: Two-photon polarization microscopy reveals protein structure and function, Nature Methods, 2011
A new type of optical microscope, developed under the leadership of researchers from the Institute of Structural Biology and nanobiology CVGZ, AS CR and University of South Bohemia, to observe living cells in various processes, which until now was not otherwise be seen.
The newly developed technology is based on an advanced type of optical microscope, the so-called two-photon microscope. For dvoufotonových microscopes biological sample is illuminated with a strong infrared laser in a way that allows three-dimensional spatial localization of fluorescent molecules (ie molecules emitting light when illuminated by light themselves on the appropriate wavelength). Fluorescent substances are present in biology are often used to visualize otherwise nebarevných biological molecules. The present improvement is used in adapting the laser beam so that its light waves oscillated alternately in different, well-defined direction (polarization). This allows you to customize dvoufotonového use the microscope to identify not only where the fluorescent molecules are located, but also how they are oriented.
Recent research has shown that the information on the orientation of fluorescent tags attached to the selected protein molecules can be inferred about the structure of protein molecules directly in living cells and tissues. Because the structure of protein molecules often changes when these molecules in cells perform an activity, the new microscope can detect whether the cell is a molecular process or not. By allowing the monitoring of processes in cells, should allow the new microscope, the discovery of new drugs that affect these processes. In addition to the pharmaceutical industry will contribute new microscope also understand how cells and whole organisms work. As a particularly interesting area of application of the new microscope appears to be able to see the electrical signals traveling in individual neurons in the brain. The layout and application of the new microscope are currently protected by the Czech patent, and efforts are made for patent protection at the international level and commercial applications.
The current publication of the results is the culmination of a ten-year effort by major publications, which began during his tenure at Columbia University in the U.S., and continued his team at the Institute of Structural Biology and nanobiology CVGZ Academy of Sciences and the University of South Bohemia, Nove Hrady and Czech Budejovice. The first three authors of published research conducted at the Academy of Sciences and University of South Bohemia, by another colleague from Columbia University. The research was financially supported by the National Institutes of Health (USA), the McKnight Foundation (USA), Columbia University (USA), the European Union, the Government of the Czech Republic, University of South Bohemia, and saving the principal investigator.
Contact: Josef Lazar, Ph.D. Institute of Structural Biology and nanobiology CVGZ of Sciences Academy of Sciences of the Czech Republic Lock 136 37333 New Castle E-mail: firstname.lastname@example.org, tel +420389033814
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